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Biomolecular biochemistry deals with the differences in the structures of different carbohydrates, which can exist in monomer or polymer formation. Lipids are the primary plasma membrane components of the cell, and there are specific phospholipids for specific purposes. Proteins and amino acids are very important Biomolecules whose structures are elucidated by x-ray crystallography.
Enzymology is another important aspect of biochemistry where the catalysis of biological reactions is studied. And the conformational changes of enzymes are studied in different Biological conditions. In an enzyme-substrate reaction, biochemists have found that enzymes also undergo conformational changes via transition states which catalyze the reaction forward.
Cellular metabolism is a congregation of all the reaction pathways that are studied by biochemists. The basic aim of metabolism is the survival and growth of the cell. This includes the generation of the energy currency ATP by electron transport pathways. And various biomolecules are oxidized at different pathways inside the cell to obtain energy.
The expression of genetic information inside the cell has opened up new research areas for biochemists. Replication of DNA for cell multiplication and transcription of the encoded DNA into RNA by specific enzymes inside the cell nucleus has been proven by the central dogma of molecular biology. RNA that contains information on protein expression is translated into proteins with the help of ribosomes in the cell cytosol.
The interaction between foreign pathogens and the antibodies and immune system cells of our body is one of the key research areas. The human body has various pathways to deal with various kinds of pathogenic organisms. And these have led scientists to discover different antidotes and vaccines against various deadly pathogens in this world.
Different kinds of molecules act as ligands and bind to proteins on the cell surface. This initiates various cytosolic reactions where cytosolic proteins get activated and work according to the signal imitated. This is a very important research area where biochemists deal with cellular signaling mechanisms.
Neurochemistry is another field of biology that is very important to biochemist researchers. It deals with the synthesis and secretion of different types of neurotransmitters and neuropeptides, and other organic compounds and their effects on the physiology of the nervous system. The cells of the nervous system are controlled by the action of neurotransmitters.
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Q.2: What is the Evaluation of Molecular Genetics in Biochemistry?
Ans: Biochemistry is the branch of biology where chemistry is applied to study the different biological processes at the cellular level. Molecular genetics deals with the differences in the structure and expression of DNA molecules and their manifestation as variations between different kinds of organisms. There are various recombinant DNA techniques that are used in molecular genetics to induce changes in genetic information in various species.
Q.3: What is the Bioseparation process in biochemistry?
Ans: Bioseparation is described as the downstream processing stages needed to purify products from bioreactors (such as fermentors). It involves extraction, precipitation, electrophoresis, and chromatography. Once the fermentor stage is complete, the process calls for cell harvesting, recovery, enzymatic conversion, solubilization, refolding, etc.
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